Journal: International Journal of Molecular Sciences

Article Title: Constant Light Dysregulates Cochlear Circadian Clock and Exacerbates Noise-Induced Hearing Loss

doi: 10.3390/ijms21207535

Figure Lengend Snippet: Synaptic ribbon counts in the LD and LL groups after 106 dB noise exposure. ( a ) The number of synaptic ribbons decreased significantly in the LD group at 32 kHz and the LL group at 16, 22.6, and 32 kHz after 106 dB noise exposure, while the significant difference between LD and LL groups was observed at 22.6 kHz. Data are mean ± SEM ( n = 5–6 for each group). ** p < 0.01 for LD + 106 dB noise vs. LD + sham, ## p < 0.01, ### p < 0.001 for LL + 106 dB noise vs. LL + sham, † p < 0.05 for LD + 106 dB noise vs. LL + 106 dB noise. ( b ) Representative images of synaptic ribbons stained by CTBP2 (green dots) over the 22.6 kHz region. Scale bar = 5 µm.

Article Snippet: After blocking with 10% donkey anti-mouse serum (Abcam, Cambridge, UK) for 30 min at room temperature (22–24 °C), the basilar membrane was immunostained with primary mouse anti-C-terminal binding protein 2 (CTBP2) IgG1 monoclonal antibody at 1:200 dilution (BD Biosciences, San Diego, CA, USA) or primary rabbit anti-4-HNE polyclonal antibody at 1:50 dilution (Abcam, Inc., Cambridge, MA, USA), and incubated at 37 °C overnight or 4 °C for 72 h, which was co-labeled with donkey anti-mouse AlexaFluor 488-conjugated secondary antibody (Abcam) at 1:1000 dilution at 37 °C for 1 h in the dark.

Techniques: Staining

Journal: Cells

Article Title: Protective Effects of SIRT6 Overexpression against DSS-Induced Colitis in Mice

doi: 10.3390/cells9061513

Figure Lengend Snippet: SIRT6 regulated expression and phosphorylation of TAK1. ( a ) The expression and phosphorylation levels of TAK1 were determined by immunohistochemistry. ( b , c ) The statistical results of ( a ). n = 3–6, ** p < 0.01.

Article Snippet: Sections then were blocked in 5% rat serum and incubated overnight at 4 °C with the diluted biotinylated primary antibody (1:500; rat-anti-mouse; anti-TAK1, #ab109526, Abcam, Cambridge, England, UK; anti-p-TAK1, #4508, Cell Signaling Technology, Danvers, MA, USA; anti-c-Jun, #ab40766, Abcam; anti-p-Jun, #ab32385, Abcam; anti-NF-κB p65 antibody, #ab32536, Abcam; anti-NF-κB p65 (phosphor S536), #ab86299, Abcam; anti-NF-κB p65 (acetyl K310) antibody, #ab19870, Abcam).

Techniques: Expressing, Immunohistochemistry